Clonal variation in Scots pine (Pinus sylvestris) and in transgenic silver birch (Betula pendula)
Niskanen A.-M. (2013). Clonal variation in Scots pine (Pinus sylvestris) and in transgenic silver birch (Betula pendula). https://doi.org/10.14214/df.157
Abstract
The aim of this study is to assess the clonal variation in Scots pine (Pinus sylvestris L.) clones and in transgenic lines of silver birch (Betula pendula Roth) and its causes, with special attention to the effects of cloning and transgenesis in tree breeding programmes. The parental effect on cloning success variation was studied in an experiment where Scots pine embryogenic lines were initiated from immature seeds of a full diallele cross. The evaluation was made after culture initiation, on maintenance medium and by mature embryo production. Growth and stem straightness of Scots pine clones were assessed in a 10-year field trial established with rooted cuttings. The effect of a single gene transfer, the sugar beet chitinase IV gene (chiIV), was assessed on plant growth, susceptibility to fungal diseases and development of root associated fungal communities and phenology, in a 3-year field trial established with micropropagated transgenic silver birch lines and wild-type clones. The results of the somatic embryogenesis experiment with Scots pine showed that the initiation success, as well as maturation, was more affected by the genotype of the mother than the one of the father, while during the proliferation period the mother s effect decreased and the father s increased. The field trial with Scots pine showed that the tree s genotype,
Keywords
clone;
variation;
gene transfer;
fungal community;
tree breeding
Published 11 April 2013
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Available at https://doi.org/10.14214/df.157 | Download PDF
Original articles
Niskanen, A-M., Lu, J., Seitz, S., Keinonen, K., von Weissenberg, K. and Pappinen, A. (2004) Effect of parent genotype on somatic embryogenesis in Scots pine (Pinus sylvestris). Tree physiology 24: 1259−1265.
https://doi.org/10.1093/treephys/24.11.1259
Niskanen, A-M., Stenvall, N., Pakkanen, A. and Pulkkinen P. (2008). Comparison of growth and stem form characters of Pinus sylvestris clones and seedlings of the same origin in a 10-year field trial. Scandinavian Journal of Forest Research 23: 484−490.
https://doi.org/10.1080/02827580802512416
Pasonen, H-L., Lu, J., Niskanen, A-M., Seppänen, S-K., Rytkönen, A., Raunio, J., Pappinen, A., Kasanen, R. and Timonen, S. (2009). Effects of sugar beet chitinase IV on root-associated fungal community of transgenic silver birch in a field trial. Planta 230: 973−983.
https://doi.org/10.1007/s00425-009-1005-4
Niskanen, A-M., Kärkkäinen, K. and Pasonen H. (2011). Comparison of variation in adaptive traits between wild-type and transgenic silver birch (Betula pendula) in a field trial. Tree Genetics & Genomes 7: 955–967.